With the EC2 serial console, you have access to your Amazon EC2 instance's serial port, which you can use to troubleshoot boot, network configuration, and other issues. The serial console does not require your instance to have any networking capabilities. With the serial console, you can enter commands to an instance as if your keyboard and monitor are directly attached to the instance's serial port. The serial console session lasts during instance reboot and stop. During reboot, you can view all of the boot messages from the start.
Access to the serial console is not available by default. Your organization must grant account access to the serial console and configure IAM policies to grant your users access to the serial console. Serial console access can be controlled at a granular level by using instance IDs, resource tags, and other IAM levers. For more information, see Configure access to the EC2 Serial Console.
Mixed in key serial
If you are moving existing data into a database, you may already have a field that you can use as the primary key. Often, a unique identification number, such as an ID number or a serial number or code, serves as a primary key in a table. For example, you might have a Customers table where each customer has a unique customer ID number. The customer ID field is the primary key.
Go beyond AC and DC theory to cover mixed signal designs with logic channels and built-in spectrum analyzer. Tek scopes have earned their reputation for reliability and are the brand of scope most often found in industry labs.
Finally, note that pg_get_serial_sequence only works if the sequence is owned by the column. This will be the case if the incrementing column was defined as a serial type, however if the sequence was added manually it is necessary to ensure ALTER SEQUENCE .. OWNED BY is also performed.
Abstract:Serial xenotransplantation of sorted cancer cells in immunodeficient mice remains the most complex test of cancer stem cell (CSC) phenotype. However, we have demonstrated in various sarcomas that putative CSC surface markers fail to identify CSCs, thereby impeding the isolation of CSCs for subsequent analyses. Here, we utilized serial xenotransplantation of unsorted rhabdomyosarcoma cells in NOD/SCID gamma (NSG) mice as a proof-of-principle platform to investigate the molecular signature of CSCs. Indeed, serial xenotransplantation steadily enriched for rhabdomyosarcoma stem-like cells characterized by enhanced aldehyde dehydrogenase activity and increased colony and sphere formation capacity in vitro. Although the expression of core pluripotency factors (SOX2, OCT4, NANOG) and common CSC markers (CD133, ABCG2, nestin) was maintained over the passages in mice, gene expression profiling revealed gradual changes in several stemness regulators and genes linked with undifferentiated myogenic precursors, e.g., SOX4, PAX3, MIR145, and CDH15. Moreover, we identified the induction of a hybrid epithelial/mesenchymal gene expression signature that was associated with the increase in CSC number. In total, 60 genes related to epithelial or mesenchymal traits were significantly altered upon serial xenotransplantation. In silico survival analysis based on the identified potential stemness-associated genes demonstrated that serial xenotransplantation of unsorted rhabdomyosarcoma cells in NSG mice might be a useful tool for the unbiased enrichment of CSCs and the identification of novel CSC-specific targets. Using this approach, we provide evidence for a recently proposed link between the hybrid epithelial/mesenchymal phenotype and cancer stemness.Keywords: rhabdomyosarcoma; cancer stem cells; stemness; stem-like state; serial xenotransplantation; in vivo tumorigenicity assay; epithelial/mesenchymal phenotype
If you turned on EC2 Serial Console for Linux, then you can use it to troubleshoot supported Nitro-based instance types. The serial console helps you troubleshoot boot issues, network configuration, and SSH configuration issues. The serial console connects to your instance without the need for a working network connection. You can access the serial console using the Amazon EC2 console or the AWS Command Line Interface (AWS CLI).
Destinations accept key-value data in standard or serialized format. Standard formatting organizes data into separate key-value pairs. Each key is stated explicitly, even when used again to define a different value. By contrast, serialized formatting condenses multiple values into one set defined by a single key. Also, in a serialized pair, a special indicator is used to separate the values within the key-value set. Finally, standard and serialized key-values can contain single or multiple values. The following table provides examples of standard and serial key-value formats.
To address this challenge, the FBI clearly used psychiatry and its bible, the DSM-III and its later version, the DSM-IV. Therein lies the origin of its well-known classification of organized serial killers, disorganized serial killers and mixed serial killers, as recognized by four of the original agents who initiated the BSU: Robert K. Ressler, John Douglas, Roy Hazelwood and Dick Ault.
The third category: that of mixed serial killers, which represented characteristics of both groups, such as the American Jeffrey Dahmer, who murdered 17 men, or the Spaniard Manuel Delgado Villegas, El Arropiero, was the last one they created.
The second type of serial killer is the disorganized, which corresponds to the psychotic, the mentally ill, almost always of a schizophrenic, paranoid or delusional type, such as Francisco García Escalero, the mendigo asesino (beggar killer), author of 10 murders in Madrid.
On many occasions, such a scene presents mixed characteristics. The crime may have begun as an organized murder and become disorganized by various factors, as happened in the case of Manuel Delgado Villegas in the Garraf crime or as happened to Edmund Kemper, a closet of more than two meters, who killed 9 people.
By default, Ansible runs each task on all hosts affected by a play before starting the next task on any host, using 5 forks. If you want to change this default behavior, you can use a different strategy plugin, change the number of forks, or apply one of several keywords like serial.
In addition to strategies, several keywords also affect play execution. You can set a number, a percentage, or a list of numbers of hosts you want to manage at a time with serial. Ansible completes the play on the specified number or percentage of hosts before starting the next batch of hosts. You can restrict the number of workers allotted to a block or task with throttle. You can control how Ansible selects the next host in a group to execute against with order. You can run a task on a single host with run_once. These keywords are not strategies. They are directives or options applied to a play, block, or task.
By default, Ansible runs in parallel against all the hosts in the pattern you set in the hosts: field of each play. If you want to manage only a few machines at a time, for example during a rolling update, you can define how many hosts Ansible should manage at a single time using the serial keyword:
Setting the batch size with serial changes the scope of the Ansible failures to the batch size, not the entire host list. You can use ignore_unreachable or max_fail_percentage to modify this behavior.
If you have already restricted the number of forks or the number of machines to execute against in parallel, you can reduce the number of workers with throttle, but you cannot increase it. In other words, to have an effect, your throttle setting must be lower than your forks or serial setting if you are using them together.
When used together with serial, tasks marked as run_once will be run on one host in each serial batch. If the task must run only once regardless of serial mode, usewhen: inventory_hostname == ansible_play_hosts_all[0] construct.
Many procedures performed in modern biology and chemistry laboratories require sets of solutions that cover a range of concentration*s. These include quantifying the number of bacteria in a sample using plate counts and the development of standard curves for quantitative colorimetric, radiometric, and enzymatic assays. Scientists perform serial dilution* to create these sets of solutions that cover a range of concentrations.
To perform a serial dilution, a small amount of a well-mixed solution is transferred into a new container, and additional water or other solvent* is added to dilute the original solution. The diluted sample is then used as the base solution to make an additional dilution. Doing this several times results in a range of concentrations.
The initial concentration and target range needed determines the size and number of dilution steps required. Serial dilutions are often performed in steps of 10 or 100. They are described as ratios of the initial and final concentrations. For example, a 1:10 dilution is a mixture of one part of a solution and nine parts fresh solvent. For a 1:100 dilution, one part of the solution is mixed with 99 parts new solvent.
Creswell, J.W., & Creswell, J.D. (2018). Mixed methods procedures. In, Research design: Qualitative, quantitative, and mixed methods approaches (5th ed., pp. 213-246). Los Angeles, CA: SAGE Publications, Inc. 2ff7e9595c
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